RAMP Microscopy

Random-access multiphoton

  • Two-photon microscopy offers the promise of monitoring activity at multiple locations within intact tissue. However, serial sampling of voxels has been difficult to reconcile with millisecond timescale of neuronal activity.
  • The recent use of acouto-optic deflector scanning to implement random-access multiphoton microscopy (RAMP) provides for long illumination dwell times while sampling points-of-interest at kHz frame rate.